Basal medium for the selective enumeration of rumen bacteria utilizing specific energy sources
نویسندگان
چکیده
منابع مشابه
Basal medium for the selective enumeration of rumen bacteria utilizing specific energy sources.
A 40% rumen fluid basal medium has been developed that without added substrate will support growth of about 10% or less of the total colony count obtained with 40% rumen fluid-glucose-cellobiose-starch-agar medium (RGCSA). The basal medium is prepared by anaerobic incubation of all ingredients in RGCSA medium except the carbohydrates, Na2CO3, and cysteine for 7 days at 38 degrees C. After incub...
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Colony counts which approximated those in a habitat-simulating, rumen fluid-agar medium (RFM) were obtained in medium 10, a medium identical to the RFM except for the replacement of rumen fluid with 1.5 x 10(-6)m hemin, 0.2% Trypticase, 0.05% yeast extract, and a 6.6 x 10(-2)m volatile fatty acid mixture qualitatively and quantitatively similar to that in rumen fluid. Single deletion of Tryptic...
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A highly specific medium was developed for the enumeration of lactate-utilizing bacteria in the rumen of sheep. This medium, which contained 2.0% lactate, 2.0% Trypticase, 0.2% yeast extract, and volatile fatty acids, hemin, and trace elements in place of rumen fluid, enabled high counts (42 x 10 to 190 x 10/g of ingesta) of lactate-utilizing bacteria to be made with a high degree of specificit...
متن کاملEnumeration and selective isolation of rumen spirochetes.
Enumeration by means of light microscopy showed that from 0.4 x 10(8) to 2.0 x 10(8) spirochetes were present per ml of bovine rumen fluid. Viable cell counts yielded slightly lower values, ranging from 0.1 x 10(8) to 1.2 x 10(8) spirochetes per ml of rumen fluid. The antibiotic rifampin, which served as a selective agent for rumen spirochetes, was added to agar media used in the estimation of ...
متن کاملVersatile medium for the enumeration of sulfate-reducing bacteria.
A lactate-yeast extract-sulfate medium, making use of both thioglycolate and ascorbate to poise the E(n) gave valid colony counts of sulfate-reducing bacteria with both pure cultures and natural samples.
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1976
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.32.5.703-710.1976